What this work is about, in plain words
An English-language abstract of the same experiment as the 2024 Tomsk abstract: in vitro, extracts of microalgae and cyanobacteria enhanced the proliferation of mesenchymal stem cells and their nitric oxide production.
Bone marrow mesenchymal stem cells are used in research on tissue repair, and new nutrient supplements are being sought for growing them. The authors tested how extracts of diatoms, cyanobacteria and green microalgae act on these cells.
Biologically active substances were extracted from the biomass into a DMSO solution. The extracts, as well as fucoxanthin and phycocyanin, were added to human and rat bone marrow stem cells. Cell proliferation was assessed with the MTT assay, and nitric oxide production with the Griess reagent.
According to the authors, the supplements enhanced cell proliferation and viability, except for the Nanofrustulum shiloi extract for human cells and phycocyanin for rat cells. The extracts and phycocyanin increased nitric oxide production by human cells in a dose-dependent manner (except N. shiloi and Leptolyngbya cf. ectocarpi); in rat cells production also rose, but without a clear dose dependence.
Limitations. A conference abstract: without figures or detailed methods. Experiments in vitro; this is not proof of an effect in the body. The same data are presented in the 2024 Tomsk conference abstract — this is not an independent confirmation. The results were obtained by the authors and do not describe Laboratory 17 materials.